Chemopreventive Efficacy of Fisetin against Benzo(a)pyrene Induced Lung Carcinogenesis in Experimental Animals Swiss Albino Mice

The present study was designed to evaluate the effects of oral administration of the fisetin on lung tumour initiation by orally applied Benzo(a)pyrene (B(a) P). Its effects on biochemical markers enzymes as well as pathological markers were evaluated. The pathological enzyme activities were increased (P<0.05) in lung cancer bearing animals when compared with control group animals. Fisetin-treated animals from groups-3 and4 showed a significant (P<0.05) decreases in the levels of these enzymes when compared to B(a)P induced group-2 animals. Levels of glycoprotein and activities of membrane ATPases play an important role in the carcinogenesis. Hence, objective this study was aimed to evaluate the effect of fisetin on the changes in glycoprotein components (hexose, hexosamine and sialic acid) and ATPases(Na + /K + ATPase, Ca 2+ ATPase and Mg 2+ ATPase) in control and lung carcinoma bearing animals. A significant increase in the levels of glycoproteins and activities of membrane ATPases were observed in animals with lung cancer. The administration of fisetin, these changes were reverted back to near normal levels of glycoproteins and membrane ATPases. Furthermore, anti-proliferative efficacy of fisetin was assessed by immunofluorescence analysis of proliferating cell nuclear antigen (PCNA) in B(a)P induced mice showed increased PCNA expression which was restored upon fisetin administration. Together, our results were depicts that fisetin can be used as a chemopreventive agent against lung cancer.